accela 600 pump (Thermo Fisher)
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accela 600 pump
Accela 600 Pump, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/accela+600+pump/accela+600+pump/10__1016_slash_j__foodcont__2025__111374-126-20-23
Average 90 stars, based on 1 article reviews
Accela 600 Pump, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/accela+600+pump/accela+600+pump/10__1016_slash_j__foodcont__2025__111374-126-20-23
Average 90 stars, based on 1 article reviews
accela 600 pump - by Bioz Stars,
2026-10
90/100 stars
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Mass Spectrometry:Article Title: Quantification of process-induced toxicants in bovine meats cooked according to the usual preparation and cooking practices in France Article Snippet: Most red meat eaten is cooked to increase its digestibility and palatability, but this cooking step can also produce process-induced toxicants (PITs).. The aim of this study was to quantify these PITs in different types of bovine meat (cuts, minced patties, and marinated meat) prepared in either home cooking, commercial foodservice, or institutional catering settings.. The sampling plan included ten types of cooked beef (six samples each) consisting of cuts that were prepared and/or cooked using methods representative of typical French practices. Article Title: A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IgG Sepharose 6 Fast Flow affinity resin Cytiva Cat# 17096901 Taq DNA polymerase This study N/A H3-H4 tetramer This study N/A H2A-H2B dimer This study N/A 3C protease This study N/A TEV protease This study N/A DTT Sigma-Aldrich Cat# 10708984001 TCEP Gold Biotechnology Cat# TECP2 BSA Thermo Scientific Cat# 23209 EPPS Sigma-Aldrich Cat# E9502 Urea Sigma-Aldrich Cat# U5378 Trypsin Promega Cat# V511C alpha-Mating Factor Pheromone, yeast Chinese peptide company Cat# SIGN-001 Paraformaldehyde (1%, w/v) Sigma-Aldrich Cat# P6148-1KG Glycine Amresco Cat# 0167-5KG BrdU Sigma-Aldrich Cat# B5002-5G Zymolyase-100T nacalai tesque Cat# 07665-84 NP-40 Thermo Fisher Scientific Cat# 28324 Nuclease, Micrococcal (MNase) Worthington Cat# LS004797 Protein G Sepharose agarose beads GE Healthcare Cat# 17061801 Chelex-100 Bio-rad Cat# 1422822 E. coli tRNA Roche Cat# 10109541001 CHAPS anatrace Cat# C316S Octyl-glucoside anatrace Cat# O311S Critical commercial assays Invitrogen SimplyBlue SafeStain Thermo Fisher Scientific Cat# LC6065 Pierce Silver Stain Kit Thermo Fisher Scientific Cat# 24612 SuperSignal West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific Cat# 34580 millTUBE 1 mL AFA fiber Covaris Cat# 520130 No. 1.5H high precision glass coverslips (24x50 mm) Thorlabs Cat# CG15KH Qiagen MinElute Kit Qiagen Cat# 28004 Accel-NGS 1S Plus DNA Library Kit for the Illumina Swift Cat# 10096 Experimental models: Organisms/strains S. pombe strains This study Table S1 S. cerevisiae strains This study Table S1 E. coli BL21-CodonPlus (DE3)-RIPL strain Agilent Cat# 230280 Oligonucleotides gRNAs for genome editing This study Table S1 qPCR Primers This study Table S1 Deposited data Immunoprecipitation-coupled mass spectrometry This study Tables S2, S3, S4, and S5 Predicted structures by AlphaFold-Multimer This study Model Archive: ma-dm-hisrep; Table S6 Raw and processed eSPAN data This study GRA Project: PRJCA018248; GRA: CRA011810; CRA014983; GEO: GSE269383 (Continued on next page) ll Cell 187, 1–19.e1–e9, September 5, 2024 e2 Please cite this article in press as: Yu et al., A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance, Cell (2024), https://doi.org/10.1016/j.cell.2024.07.006 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms UCSF Chimera X daily build (2022-10-26) version UCSF Chimera X RRID:SCR_015872 ColabFold Google Colab N/A localColabFold Harvard Medical School O2 computing cluster N/A In-house mass spectrometry data analysis software Gibson et al.110 N/A ChatGPT3.5 (March 24 version) OpenAI RRID:SCR_023775 Clustal Omega UniProt RRID:SCR_001591 JalView University of Dundee RRID:SCR_006459 AcquireMP Refeyn, Ltd N/A DiscoverMP Refeyn, Ltd N/A ASTRA, version 7.3.2.21 Wyatt RRID:SCR_016255 Bowtie2 John Hopkins University RRID:SCR_016368 MACS Dana Farber Cancer Institute RRID:SCR_013291 DANPOS Baylor College of Medicine RRID:SCR_015527 Raw data This study Mendeley data: 10.17632/jhzmfr8bbs.1 Other MagNA Lyser Instrument Roche Cat# 3358968001 QuantStudio 7 Flex Real-Time PCR System, 384-well, desktop Applied Bioystems Cat# 4485701 6875 Freezer/Mill High Capacity Cryogenic Grinder SPEXSamplePrep Cat# 6875 E220evolution Focused-ultrasonicator Covaris Cat# 500429 Q Exactive HF-X Hybrid QuadrupoleOrbitrap MS System Thermo Fisher Scientific Cat# 0726042 Article Title: Dietary product devoid of at least two non essential amino acids Article Snippet: .. Liquid Chromatography-Mass Spectrometry: Samples were shaken at 4° C. for 10 min, then centrifuged for 15 min at 16,000×g, and the supernatant was collected and analyzed by LC-MS. Analytes were separated using hydrophilic interaction liquid chromatography with a SeQuant ZIC-pHILIC column (2.1×150 mm, 5 μm) (Merck) and detected with high-resolution, accurate-mass mass spectrometry using an Orbitrap Exactive in line with an Accela autosampler and an other:Article Title: Evaluation and assessment of risk of pesticide residue in vegetables, soil and water samples of few district of Northern India Article Snippet: The samples was analysed using Article Title: Macrophage-derived insulin antagonist ImpL2 induces lipoprotein mobilization upon bacterial infection. Article Snippet: Briefly, high-performance liquid chromatography (Accela 600 pump, Accela AS autosampler) combined with Article Title: A new adsorptive 3D-printed sampling device for simultaneous determination of 63 urinary organic acids by LC-MS/MS. Article Snippet: • Quantification of urinary metabolites is used for disease diagnosis.. • Dried urine sampling devices minimize analyte degradation.. • The device is effective for simultaneous preservation of 63 urinary organic acids. Liquid Chromatography:Article Title: Monitoring and risk assessment for pesticide residues in vegetables, soil, and water in Haryana, India. Article Snippet: The present study was conducted with the aim to identify estimates and risk assessment for the pesticide residue present in vegetables, soil, and water in Haryana, India.. In the present study, environmental monitoring of commonly sprayed 161 pesticides was done using liquid chromatography-tandem mass spectrometry and gas chromatography-tandem mass spectrometry for 102 vegetables, 46 soil, and 50 water samples collected from four districts in Haryana, India.. A total of 198 samples were analyzed for the presence of pesticide residue and the measured pesticide concentrations were compared with the Maximum Residue Limit (MRL) set by the Food Safety and Standards Authority of India (FSSAI) and European regulations. Targeted Proteomics:Article Title: Monitoring and risk assessment for pesticide residues in vegetables, soil, and water in Haryana, India. Article Snippet: The present study was conducted with the aim to identify estimates and risk assessment for the pesticide residue present in vegetables, soil, and water in Haryana, India.. In the present study, environmental monitoring of commonly sprayed 161 pesticides was done using liquid chromatography-tandem mass spectrometry and gas chromatography-tandem mass spectrometry for 102 vegetables, 46 soil, and 50 water samples collected from four districts in Haryana, India.. A total of 198 samples were analyzed for the presence of pesticide residue and the measured pesticide concentrations were compared with the Maximum Residue Limit (MRL) set by the Food Safety and Standards Authority of India (FSSAI) and European regulations. Software:Article Title: A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IgG Sepharose 6 Fast Flow affinity resin Cytiva Cat# 17096901 Taq DNA polymerase This study N/A H3-H4 tetramer This study N/A H2A-H2B dimer This study N/A 3C protease This study N/A TEV protease This study N/A DTT Sigma-Aldrich Cat# 10708984001 TCEP Gold Biotechnology Cat# TECP2 BSA Thermo Scientific Cat# 23209 EPPS Sigma-Aldrich Cat# E9502 Urea Sigma-Aldrich Cat# U5378 Trypsin Promega Cat# V511C alpha-Mating Factor Pheromone, yeast Chinese peptide company Cat# SIGN-001 Paraformaldehyde (1%, w/v) Sigma-Aldrich Cat# P6148-1KG Glycine Amresco Cat# 0167-5KG BrdU Sigma-Aldrich Cat# B5002-5G Zymolyase-100T nacalai tesque Cat# 07665-84 NP-40 Thermo Fisher Scientific Cat# 28324 Nuclease, Micrococcal (MNase) Worthington Cat# LS004797 Protein G Sepharose agarose beads GE Healthcare Cat# 17061801 Chelex-100 Bio-rad Cat# 1422822 E. coli tRNA Roche Cat# 10109541001 CHAPS anatrace Cat# C316S Octyl-glucoside anatrace Cat# O311S Critical commercial assays Invitrogen SimplyBlue SafeStain Thermo Fisher Scientific Cat# LC6065 Pierce Silver Stain Kit Thermo Fisher Scientific Cat# 24612 SuperSignal West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific Cat# 34580 millTUBE 1 mL AFA fiber Covaris Cat# 520130 No. 1.5H high precision glass coverslips (24x50 mm) Thorlabs Cat# CG15KH Qiagen MinElute Kit Qiagen Cat# 28004 Accel-NGS 1S Plus DNA Library Kit for the Illumina Swift Cat# 10096 Experimental models: Organisms/strains S. pombe strains This study Table S1 S. cerevisiae strains This study Table S1 E. coli BL21-CodonPlus (DE3)-RIPL strain Agilent Cat# 230280 Oligonucleotides gRNAs for genome editing This study Table S1 qPCR Primers This study Table S1 Deposited data Immunoprecipitation-coupled mass spectrometry This study Tables S2, S3, S4, and S5 Predicted structures by AlphaFold-Multimer This study Model Archive: ma-dm-hisrep; Table S6 Raw and processed eSPAN data This study GRA Project: PRJCA018248; GRA: CRA011810; CRA014983; GEO: GSE269383 (Continued on next page) ll Cell 187, 1–19.e1–e9, September 5, 2024 e2 Please cite this article in press as: Yu et al., A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance, Cell (2024), https://doi.org/10.1016/j.cell.2024.07.006 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms UCSF Chimera X daily build (2022-10-26) version UCSF Chimera X RRID:SCR_015872 ColabFold Google Colab N/A localColabFold Harvard Medical School O2 computing cluster N/A In-house mass spectrometry data analysis software Gibson et al.110 N/A ChatGPT3.5 (March 24 version) OpenAI RRID:SCR_023775 Clustal Omega UniProt RRID:SCR_001591 JalView University of Dundee RRID:SCR_006459 AcquireMP Refeyn, Ltd N/A DiscoverMP Refeyn, Ltd N/A ASTRA, version 7.3.2.21 Wyatt RRID:SCR_016255 Bowtie2 John Hopkins University RRID:SCR_016368 MACS Dana Farber Cancer Institute RRID:SCR_013291 DANPOS Baylor College of Medicine RRID:SCR_015527 Raw data This study Mendeley data: 10.17632/jhzmfr8bbs.1 Other MagNA Lyser Instrument Roche Cat# 3358968001 QuantStudio 7 Flex Real-Time PCR System, 384-well, desktop Applied Bioystems Cat# 4485701 6875 Freezer/Mill High Capacity Cryogenic Grinder SPEXSamplePrep Cat# 6875 E220evolution Focused-ultrasonicator Covaris Cat# 500429 Q Exactive HF-X Hybrid QuadrupoleOrbitrap MS System Thermo Fisher Scientific Cat# 0726042 Magnetic Cell Separation:Article Title: A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IgG Sepharose 6 Fast Flow affinity resin Cytiva Cat# 17096901 Taq DNA polymerase This study N/A H3-H4 tetramer This study N/A H2A-H2B dimer This study N/A 3C protease This study N/A TEV protease This study N/A DTT Sigma-Aldrich Cat# 10708984001 TCEP Gold Biotechnology Cat# TECP2 BSA Thermo Scientific Cat# 23209 EPPS Sigma-Aldrich Cat# E9502 Urea Sigma-Aldrich Cat# U5378 Trypsin Promega Cat# V511C alpha-Mating Factor Pheromone, yeast Chinese peptide company Cat# SIGN-001 Paraformaldehyde (1%, w/v) Sigma-Aldrich Cat# P6148-1KG Glycine Amresco Cat# 0167-5KG BrdU Sigma-Aldrich Cat# B5002-5G Zymolyase-100T nacalai tesque Cat# 07665-84 NP-40 Thermo Fisher Scientific Cat# 28324 Nuclease, Micrococcal (MNase) Worthington Cat# LS004797 Protein G Sepharose agarose beads GE Healthcare Cat# 17061801 Chelex-100 Bio-rad Cat# 1422822 E. coli tRNA Roche Cat# 10109541001 CHAPS anatrace Cat# C316S Octyl-glucoside anatrace Cat# O311S Critical commercial assays Invitrogen SimplyBlue SafeStain Thermo Fisher Scientific Cat# LC6065 Pierce Silver Stain Kit Thermo Fisher Scientific Cat# 24612 SuperSignal West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific Cat# 34580 millTUBE 1 mL AFA fiber Covaris Cat# 520130 No. 1.5H high precision glass coverslips (24x50 mm) Thorlabs Cat# CG15KH Qiagen MinElute Kit Qiagen Cat# 28004 Accel-NGS 1S Plus DNA Library Kit for the Illumina Swift Cat# 10096 Experimental models: Organisms/strains S. pombe strains This study Table S1 S. cerevisiae strains This study Table S1 E. coli BL21-CodonPlus (DE3)-RIPL strain Agilent Cat# 230280 Oligonucleotides gRNAs for genome editing This study Table S1 qPCR Primers This study Table S1 Deposited data Immunoprecipitation-coupled mass spectrometry This study Tables S2, S3, S4, and S5 Predicted structures by AlphaFold-Multimer This study Model Archive: ma-dm-hisrep; Table S6 Raw and processed eSPAN data This study GRA Project: PRJCA018248; GRA: CRA011810; CRA014983; GEO: GSE269383 (Continued on next page) ll Cell 187, 1–19.e1–e9, September 5, 2024 e2 Please cite this article in press as: Yu et al., A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance, Cell (2024), https://doi.org/10.1016/j.cell.2024.07.006 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms UCSF Chimera X daily build (2022-10-26) version UCSF Chimera X RRID:SCR_015872 ColabFold Google Colab N/A localColabFold Harvard Medical School O2 computing cluster N/A In-house mass spectrometry data analysis software Gibson et al.110 N/A ChatGPT3.5 (March 24 version) OpenAI RRID:SCR_023775 Clustal Omega UniProt RRID:SCR_001591 JalView University of Dundee RRID:SCR_006459 AcquireMP Refeyn, Ltd N/A DiscoverMP Refeyn, Ltd N/A ASTRA, version 7.3.2.21 Wyatt RRID:SCR_016255 Bowtie2 John Hopkins University RRID:SCR_016368 MACS Dana Farber Cancer Institute RRID:SCR_013291 DANPOS Baylor College of Medicine RRID:SCR_015527 Raw data This study Mendeley data: 10.17632/jhzmfr8bbs.1 Other MagNA Lyser Instrument Roche Cat# 3358968001 QuantStudio 7 Flex Real-Time PCR System, 384-well, desktop Applied Bioystems Cat# 4485701 6875 Freezer/Mill High Capacity Cryogenic Grinder SPEXSamplePrep Cat# 6875 E220evolution Focused-ultrasonicator Covaris Cat# 500429 Q Exactive HF-X Hybrid QuadrupoleOrbitrap MS System Thermo Fisher Scientific Cat# 0726042 Real-time Polymerase Chain Reaction:Article Title: A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IgG Sepharose 6 Fast Flow affinity resin Cytiva Cat# 17096901 Taq DNA polymerase This study N/A H3-H4 tetramer This study N/A H2A-H2B dimer This study N/A 3C protease This study N/A TEV protease This study N/A DTT Sigma-Aldrich Cat# 10708984001 TCEP Gold Biotechnology Cat# TECP2 BSA Thermo Scientific Cat# 23209 EPPS Sigma-Aldrich Cat# E9502 Urea Sigma-Aldrich Cat# U5378 Trypsin Promega Cat# V511C alpha-Mating Factor Pheromone, yeast Chinese peptide company Cat# SIGN-001 Paraformaldehyde (1%, w/v) Sigma-Aldrich Cat# P6148-1KG Glycine Amresco Cat# 0167-5KG BrdU Sigma-Aldrich Cat# B5002-5G Zymolyase-100T nacalai tesque Cat# 07665-84 NP-40 Thermo Fisher Scientific Cat# 28324 Nuclease, Micrococcal (MNase) Worthington Cat# LS004797 Protein G Sepharose agarose beads GE Healthcare Cat# 17061801 Chelex-100 Bio-rad Cat# 1422822 E. coli tRNA Roche Cat# 10109541001 CHAPS anatrace Cat# C316S Octyl-glucoside anatrace Cat# O311S Critical commercial assays Invitrogen SimplyBlue SafeStain Thermo Fisher Scientific Cat# LC6065 Pierce Silver Stain Kit Thermo Fisher Scientific Cat# 24612 SuperSignal West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific Cat# 34580 millTUBE 1 mL AFA fiber Covaris Cat# 520130 No. 1.5H high precision glass coverslips (24x50 mm) Thorlabs Cat# CG15KH Qiagen MinElute Kit Qiagen Cat# 28004 Accel-NGS 1S Plus DNA Library Kit for the Illumina Swift Cat# 10096 Experimental models: Organisms/strains S. pombe strains This study Table S1 S. cerevisiae strains This study Table S1 E. coli BL21-CodonPlus (DE3)-RIPL strain Agilent Cat# 230280 Oligonucleotides gRNAs for genome editing This study Table S1 qPCR Primers This study Table S1 Deposited data Immunoprecipitation-coupled mass spectrometry This study Tables S2, S3, S4, and S5 Predicted structures by AlphaFold-Multimer This study Model Archive: ma-dm-hisrep; Table S6 Raw and processed eSPAN data This study GRA Project: PRJCA018248; GRA: CRA011810; CRA014983; GEO: GSE269383 (Continued on next page) ll Cell 187, 1–19.e1–e9, September 5, 2024 e2 Please cite this article in press as: Yu et al., A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance, Cell (2024), https://doi.org/10.1016/j.cell.2024.07.006 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms UCSF Chimera X daily build (2022-10-26) version UCSF Chimera X RRID:SCR_015872 ColabFold Google Colab N/A localColabFold Harvard Medical School O2 computing cluster N/A In-house mass spectrometry data analysis software Gibson et al.110 N/A ChatGPT3.5 (March 24 version) OpenAI RRID:SCR_023775 Clustal Omega UniProt RRID:SCR_001591 JalView University of Dundee RRID:SCR_006459 AcquireMP Refeyn, Ltd N/A DiscoverMP Refeyn, Ltd N/A ASTRA, version 7.3.2.21 Wyatt RRID:SCR_016255 Bowtie2 John Hopkins University RRID:SCR_016368 MACS Dana Farber Cancer Institute RRID:SCR_013291 DANPOS Baylor College of Medicine RRID:SCR_015527 Raw data This study Mendeley data: 10.17632/jhzmfr8bbs.1 Other MagNA Lyser Instrument Roche Cat# 3358968001 QuantStudio 7 Flex Real-Time PCR System, 384-well, desktop Applied Bioystems Cat# 4485701 6875 Freezer/Mill High Capacity Cryogenic Grinder SPEXSamplePrep Cat# 6875 E220evolution Focused-ultrasonicator Covaris Cat# 500429 Q Exactive HF-X Hybrid QuadrupoleOrbitrap MS System Thermo Fisher Scientific Cat# 0726042 High Performance Liquid Chromatography:Article Title: A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IgG Sepharose 6 Fast Flow affinity resin Cytiva Cat# 17096901 Taq DNA polymerase This study N/A H3-H4 tetramer This study N/A H2A-H2B dimer This study N/A 3C protease This study N/A TEV protease This study N/A DTT Sigma-Aldrich Cat# 10708984001 TCEP Gold Biotechnology Cat# TECP2 BSA Thermo Scientific Cat# 23209 EPPS Sigma-Aldrich Cat# E9502 Urea Sigma-Aldrich Cat# U5378 Trypsin Promega Cat# V511C alpha-Mating Factor Pheromone, yeast Chinese peptide company Cat# SIGN-001 Paraformaldehyde (1%, w/v) Sigma-Aldrich Cat# P6148-1KG Glycine Amresco Cat# 0167-5KG BrdU Sigma-Aldrich Cat# B5002-5G Zymolyase-100T nacalai tesque Cat# 07665-84 NP-40 Thermo Fisher Scientific Cat# 28324 Nuclease, Micrococcal (MNase) Worthington Cat# LS004797 Protein G Sepharose agarose beads GE Healthcare Cat# 17061801 Chelex-100 Bio-rad Cat# 1422822 E. coli tRNA Roche Cat# 10109541001 CHAPS anatrace Cat# C316S Octyl-glucoside anatrace Cat# O311S Critical commercial assays Invitrogen SimplyBlue SafeStain Thermo Fisher Scientific Cat# LC6065 Pierce Silver Stain Kit Thermo Fisher Scientific Cat# 24612 SuperSignal West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific Cat# 34580 millTUBE 1 mL AFA fiber Covaris Cat# 520130 No. 1.5H high precision glass coverslips (24x50 mm) Thorlabs Cat# CG15KH Qiagen MinElute Kit Qiagen Cat# 28004 Accel-NGS 1S Plus DNA Library Kit for the Illumina Swift Cat# 10096 Experimental models: Organisms/strains S. pombe strains This study Table S1 S. cerevisiae strains This study Table S1 E. coli BL21-CodonPlus (DE3)-RIPL strain Agilent Cat# 230280 Oligonucleotides gRNAs for genome editing This study Table S1 qPCR Primers This study Table S1 Deposited data Immunoprecipitation-coupled mass spectrometry This study Tables S2, S3, S4, and S5 Predicted structures by AlphaFold-Multimer This study Model Archive: ma-dm-hisrep; Table S6 Raw and processed eSPAN data This study GRA Project: PRJCA018248; GRA: CRA011810; CRA014983; GEO: GSE269383 (Continued on next page) ll Cell 187, 1–19.e1–e9, September 5, 2024 e2 Please cite this article in press as: Yu et al., A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance, Cell (2024), https://doi.org/10.1016/j.cell.2024.07.006 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms UCSF Chimera X daily build (2022-10-26) version UCSF Chimera X RRID:SCR_015872 ColabFold Google Colab N/A localColabFold Harvard Medical School O2 computing cluster N/A In-house mass spectrometry data analysis software Gibson et al.110 N/A ChatGPT3.5 (March 24 version) OpenAI RRID:SCR_023775 Clustal Omega UniProt RRID:SCR_001591 JalView University of Dundee RRID:SCR_006459 AcquireMP Refeyn, Ltd N/A DiscoverMP Refeyn, Ltd N/A ASTRA, version 7.3.2.21 Wyatt RRID:SCR_016255 Bowtie2 John Hopkins University RRID:SCR_016368 MACS Dana Farber Cancer Institute RRID:SCR_013291 DANPOS Baylor College of Medicine RRID:SCR_015527 Raw data This study Mendeley data: 10.17632/jhzmfr8bbs.1 Other MagNA Lyser Instrument Roche Cat# 3358968001 QuantStudio 7 Flex Real-Time PCR System, 384-well, desktop Applied Bioystems Cat# 4485701 6875 Freezer/Mill High Capacity Cryogenic Grinder SPEXSamplePrep Cat# 6875 E220evolution Focused-ultrasonicator Covaris Cat# 500429 Q Exactive HF-X Hybrid QuadrupoleOrbitrap MS System Thermo Fisher Scientific Cat# 0726042 Multi-Angle Light Scattering:Article Title: A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IgG Sepharose 6 Fast Flow affinity resin Cytiva Cat# 17096901 Taq DNA polymerase This study N/A H3-H4 tetramer This study N/A H2A-H2B dimer This study N/A 3C protease This study N/A TEV protease This study N/A DTT Sigma-Aldrich Cat# 10708984001 TCEP Gold Biotechnology Cat# TECP2 BSA Thermo Scientific Cat# 23209 EPPS Sigma-Aldrich Cat# E9502 Urea Sigma-Aldrich Cat# U5378 Trypsin Promega Cat# V511C alpha-Mating Factor Pheromone, yeast Chinese peptide company Cat# SIGN-001 Paraformaldehyde (1%, w/v) Sigma-Aldrich Cat# P6148-1KG Glycine Amresco Cat# 0167-5KG BrdU Sigma-Aldrich Cat# B5002-5G Zymolyase-100T nacalai tesque Cat# 07665-84 NP-40 Thermo Fisher Scientific Cat# 28324 Nuclease, Micrococcal (MNase) Worthington Cat# LS004797 Protein G Sepharose agarose beads GE Healthcare Cat# 17061801 Chelex-100 Bio-rad Cat# 1422822 E. coli tRNA Roche Cat# 10109541001 CHAPS anatrace Cat# C316S Octyl-glucoside anatrace Cat# O311S Critical commercial assays Invitrogen SimplyBlue SafeStain Thermo Fisher Scientific Cat# LC6065 Pierce Silver Stain Kit Thermo Fisher Scientific Cat# 24612 SuperSignal West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific Cat# 34580 millTUBE 1 mL AFA fiber Covaris Cat# 520130 No. 1.5H high precision glass coverslips (24x50 mm) Thorlabs Cat# CG15KH Qiagen MinElute Kit Qiagen Cat# 28004 Accel-NGS 1S Plus DNA Library Kit for the Illumina Swift Cat# 10096 Experimental models: Organisms/strains S. pombe strains This study Table S1 S. cerevisiae strains This study Table S1 E. coli BL21-CodonPlus (DE3)-RIPL strain Agilent Cat# 230280 Oligonucleotides gRNAs for genome editing This study Table S1 qPCR Primers This study Table S1 Deposited data Immunoprecipitation-coupled mass spectrometry This study Tables S2, S3, S4, and S5 Predicted structures by AlphaFold-Multimer This study Model Archive: ma-dm-hisrep; Table S6 Raw and processed eSPAN data This study GRA Project: PRJCA018248; GRA: CRA011810; CRA014983; GEO: GSE269383 (Continued on next page) ll Cell 187, 1–19.e1–e9, September 5, 2024 e2 Please cite this article in press as: Yu et al., A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance, Cell (2024), https://doi.org/10.1016/j.cell.2024.07.006 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms UCSF Chimera X daily build (2022-10-26) version UCSF Chimera X RRID:SCR_015872 ColabFold Google Colab N/A localColabFold Harvard Medical School O2 computing cluster N/A In-house mass spectrometry data analysis software Gibson et al.110 N/A ChatGPT3.5 (March 24 version) OpenAI RRID:SCR_023775 Clustal Omega UniProt RRID:SCR_001591 JalView University of Dundee RRID:SCR_006459 AcquireMP Refeyn, Ltd N/A DiscoverMP Refeyn, Ltd N/A ASTRA, version 7.3.2.21 Wyatt RRID:SCR_016255 Bowtie2 John Hopkins University RRID:SCR_016368 MACS Dana Farber Cancer Institute RRID:SCR_013291 DANPOS Baylor College of Medicine RRID:SCR_015527 Raw data This study Mendeley data: 10.17632/jhzmfr8bbs.1 Other MagNA Lyser Instrument Roche Cat# 3358968001 QuantStudio 7 Flex Real-Time PCR System, 384-well, desktop Applied Bioystems Cat# 4485701 6875 Freezer/Mill High Capacity Cryogenic Grinder SPEXSamplePrep Cat# 6875 E220evolution Focused-ultrasonicator Covaris Cat# 500429 Q Exactive HF-X Hybrid QuadrupoleOrbitrap MS System Thermo Fisher Scientific Cat# 0726042 Refractive Index:Article Title: A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IgG Sepharose 6 Fast Flow affinity resin Cytiva Cat# 17096901 Taq DNA polymerase This study N/A H3-H4 tetramer This study N/A H2A-H2B dimer This study N/A 3C protease This study N/A TEV protease This study N/A DTT Sigma-Aldrich Cat# 10708984001 TCEP Gold Biotechnology Cat# TECP2 BSA Thermo Scientific Cat# 23209 EPPS Sigma-Aldrich Cat# E9502 Urea Sigma-Aldrich Cat# U5378 Trypsin Promega Cat# V511C alpha-Mating Factor Pheromone, yeast Chinese peptide company Cat# SIGN-001 Paraformaldehyde (1%, w/v) Sigma-Aldrich Cat# P6148-1KG Glycine Amresco Cat# 0167-5KG BrdU Sigma-Aldrich Cat# B5002-5G Zymolyase-100T nacalai tesque Cat# 07665-84 NP-40 Thermo Fisher Scientific Cat# 28324 Nuclease, Micrococcal (MNase) Worthington Cat# LS004797 Protein G Sepharose agarose beads GE Healthcare Cat# 17061801 Chelex-100 Bio-rad Cat# 1422822 E. coli tRNA Roche Cat# 10109541001 CHAPS anatrace Cat# C316S Octyl-glucoside anatrace Cat# O311S Critical commercial assays Invitrogen SimplyBlue SafeStain Thermo Fisher Scientific Cat# LC6065 Pierce Silver Stain Kit Thermo Fisher Scientific Cat# 24612 SuperSignal West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific Cat# 34580 millTUBE 1 mL AFA fiber Covaris Cat# 520130 No. 1.5H high precision glass coverslips (24x50 mm) Thorlabs Cat# CG15KH Qiagen MinElute Kit Qiagen Cat# 28004 Accel-NGS 1S Plus DNA Library Kit for the Illumina Swift Cat# 10096 Experimental models: Organisms/strains S. pombe strains This study Table S1 S. cerevisiae strains This study Table S1 E. coli BL21-CodonPlus (DE3)-RIPL strain Agilent Cat# 230280 Oligonucleotides gRNAs for genome editing This study Table S1 qPCR Primers This study Table S1 Deposited data Immunoprecipitation-coupled mass spectrometry This study Tables S2, S3, S4, and S5 Predicted structures by AlphaFold-Multimer This study Model Archive: ma-dm-hisrep; Table S6 Raw and processed eSPAN data This study GRA Project: PRJCA018248; GRA: CRA011810; CRA014983; GEO: GSE269383 (Continued on next page) ll Cell 187, 1–19.e1–e9, September 5, 2024 e2 Please cite this article in press as: Yu et al., A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance, Cell (2024), https://doi.org/10.1016/j.cell.2024.07.006 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms UCSF Chimera X daily build (2022-10-26) version UCSF Chimera X RRID:SCR_015872 ColabFold Google Colab N/A localColabFold Harvard Medical School O2 computing cluster N/A In-house mass spectrometry data analysis software Gibson et al.110 N/A ChatGPT3.5 (March 24 version) OpenAI RRID:SCR_023775 Clustal Omega UniProt RRID:SCR_001591 JalView University of Dundee RRID:SCR_006459 AcquireMP Refeyn, Ltd N/A DiscoverMP Refeyn, Ltd N/A ASTRA, version 7.3.2.21 Wyatt RRID:SCR_016255 Bowtie2 John Hopkins University RRID:SCR_016368 MACS Dana Farber Cancer Institute RRID:SCR_013291 DANPOS Baylor College of Medicine RRID:SCR_015527 Raw data This study Mendeley data: 10.17632/jhzmfr8bbs.1 Other MagNA Lyser Instrument Roche Cat# 3358968001 QuantStudio 7 Flex Real-Time PCR System, 384-well, desktop Applied Bioystems Cat# 4485701 6875 Freezer/Mill High Capacity Cryogenic Grinder SPEXSamplePrep Cat# 6875 E220evolution Focused-ultrasonicator Covaris Cat# 500429 Q Exactive HF-X Hybrid QuadrupoleOrbitrap MS System Thermo Fisher Scientific Cat# 0726042 Chromatography:Article Title: Dietary product devoid of at least two non essential amino acids Article Snippet: .. Liquid Chromatography-Mass Spectrometry: Samples were shaken at 4° C. for 10 min, then centrifuged for 15 min at 16,000×g, and the supernatant was collected and analyzed by LC-MS. Analytes were separated using hydrophilic interaction liquid chromatography with a SeQuant ZIC-pHILIC column (2.1×150 mm, 5 μm) (Merck) and detected with high-resolution, accurate-mass mass spectrometry using an Orbitrap Exactive in line with an Accela autosampler and an Hydrophilic Interaction Liquid Chromatography:Article Title: Dietary product devoid of at least two non essential amino acids Article Snippet: .. Liquid Chromatography-Mass Spectrometry: Samples were shaken at 4° C. for 10 min, then centrifuged for 15 min at 16,000×g, and the supernatant was collected and analyzed by LC-MS. Analytes were separated using hydrophilic interaction liquid chromatography with a SeQuant ZIC-pHILIC column (2.1×150 mm, 5 μm) (Merck) and detected with high-resolution, accurate-mass mass spectrometry using an Orbitrap Exactive in line with an Accela autosampler and an |